Table 2 Primary plasmids used in this study
Name | Description | Reference |
|---|---|---|
YLEP-Leu/nat | Y. lipolytica episomal vector, ut8 promoter with CYC1 terminator, Leu2 or nourseothricin selection marker | [47] |
pki-2/nat | Y. lipolytica integrative vector, hp4d promoter with XPR2 terminator, TEF promoter with CYC1 terminator, URA3 or nourseothricin selection marker | [12] |
YLEP-Leu-Aan | YLEP-Leu vector containing codon-optimized AanFS under ut8 promoter | This study |
YLEP-Leu-Him | YLEP-Leu vector containing codon-optimized HimFS under ut8 promoter | This study |
YLEP-Leu-Mas | YLEP-Leu vector containing codon-optimized MasFS under ut8 promoter | This study |
YLEP-Leu-Pme | YLEP-Leu vector containing codon-optimized PmeFS under ut8 promoter | This study |
pET28a-Aan | pET28a vector containing codon-optimized AanFS under T7 promoter | This study |
pET28-mAan | pET28a vector containing codon-optimized AanFSK197T/F180H under T7 promoter | This study |
YLEP-Leu-Aan-ERG20 | YLEP-Leu vector containing AanFS under ut8 promoter and GPD-ERG20-XPR2 fragments | This study |
YLEP-Leu-AanERG20 | YLEP-Leu vector containing fusion gene of codon-optimized AanFS and ERG20 (AanFSERG20) with GGGGS linker and ERG20 is at the 3' end | This study |
YLEP-Leu-ERG20Aan | YLEP-Leu vector containing fusion gene of codon-optimized AanFS and ERG20 (ERG20AanFS) with GGGGS linker and AanFS is at the 3' end | This study |
YLEP-Leu-ERG20mAan | YLEP-Leu-ERG20Aan vector with the K197T/F180H mutation in AanFS | This study |
pki-ERG12-IDI-ERG20mAan | pki-2 vector containing ERG20FSK197T/F180H under hp4d promoter, ERG12 under ut8 promoter and TEF-IDI-CYC1 fragments | This study |
114-GPPS-ERG8-ERG19 | 114-EXP-FBA vector containing GPPS under EXP promoter, GPD-ERG8-XPR2 and TEF-ERG19-CYC1 fragments | This study |
pki-AtoB-HMGR-HMGS | pKi-2 vector containing codon-optimized AtoB under TEF promoter, HMGS under hp4d promoter, and ut8-HMGR-CYC1 fragments | This study |
pki-AtoB-HMGR-HMGS-ePTS1 | The ePTS1 peroxisome localization signal was fused at C-terminal of AtoB, HMGR and HMGS separately of pki-AtoB-HMGR-HMGS plasmid | This study |
YLEP-nat-FAA1 | YLEP-nat vector containing FAA1 gene under ut8 promoter | This study |
YLEP-nat-MFE2 | YLEP-nat vector containing MFE1 gene under ut8 promoter | This study |
YLEP-nat-PAT1 | YLEP-nat vector containing PAT1 gene under ut8 promoter | This study |
YLEP-nat-PEX10 | YLEP-nat vector containing PEX10 gene under ut8 promoter | This study |
YLEP-nat-POT1 | YLEP-nat vector containing POT1 gene under ut8 promoter | This study |
pki-nat-PXA1/2 | pki-nat vector containing PXA1 and PXA2 genes | This study |
pki-nat-TGL3/4 | pki-nat vector containing TGL3 and TGL4 genes | This study |
pki-nat-POX2/3 | pki-nat vector containing POX2 and POX3 genes | This study |
pki-nat-YHM2-AMPD | pki-nat vector containing AMPD and YHM2 genes | This study |
pki-Bleor-ACL1/2 | pki-Bleor vector containing ACL1 and ACL2 genes with bleomycin selection marker | This study |
pCAS1yl-gDGA1 | pCAS1yl plasmid with gRNA of DGA1 and bleomycin selection marker | This study |
pCAS1yl-gDGA2 | pCAS1yl plasmid with gRNA of DGA2 and bleomycin selection marker | This study |
pCAS1yl-gDGA2 | pCAS1yl plasmid with gRNA of DGA2 and bleomycin selection marker | This study |