Fig. 7

CEF3 is involved in endocytic pathway. A, B Confocal microscopy images showing the internalization of FM4–64 in root cells of WT and cef3 at 0 min (A) and 30 min (B), FM4-64 signals within 0 min (A) and 30 min (B) of treatment. The cef3 mutant reduces the internalization of FM4-64. C Quantitative analyses of FM4–64 fluorescence intensity ratio of intracellular and PM in (B) (n = 30). Error bars indicate SE from the mean of three replicates. Different letters denote significant differences (P < 0.05, Duncan’s multiple range test)