Fig. 9From: CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in ricecef3 mutation affects the abundance of OsCESA9 at the plasma membrane. A Western blotting of different fractions of OsCESA9 proteins with anti-Flag antibodies. TM, total membranes; PEG, the plasma membrane fraction; DEX, the endomembrane fraction; Anti-HSP90, internal control; Anti-BiP2 and Anti-PIP1s antibodies are used to label the marker protein in the endomembrane and plasma membrane, respectively. Western blotting analysis has been repeated for at least 3 times. B Quantification of the relative chemiluminescent signal intensity shown in (A). Intensity detected in wild-type plants is set to 1 (means of three biological replicates). Error bars indicate SE from the mean of three replicates. ** indicate P < 0.01 (t test, compared with the WT value)Back to article page