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Fig. 9 | Biotechnology for Biofuels and Bioproducts

Fig. 9

From: CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice

Fig. 9

cef3 mutation affects the abundance of OsCESA9 at the plasma membrane. A Western blotting of different fractions of OsCESA9 proteins with anti-Flag antibodies. TM, total membranes; PEG, the plasma membrane fraction; DEX, the endomembrane fraction; Anti-HSP90, internal control; Anti-BiP2 and Anti-PIP1s antibodies are used to label the marker protein in the endomembrane and plasma membrane, respectively. Western blotting analysis has been repeated for at least 3 times. B Quantification of the relative chemiluminescent signal intensity shown in (A). Intensity detected in wild-type plants is set to 1 (means of three biological replicates). Error bars indicate SE from the mean of three replicates. ** indicate P < 0.01 (t test, compared with the WT value)

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