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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Metabolic recycling of storage lipids promotes squalene biosynthesis in yeast

Fig. 5

Effect of the carbon source and cultivation medium on squalene production in the squalene-overproducing strain SQ4td-TGL3. Analysis of the SQ4td-TGL3 strain for the production of squalene using three different carbon sources in YSC minimal or YP complex medium. The highest squalene titer was achieved in YP complex medium containing 2% (w/v) glucose at 144 h of cultivation. The strain was cultivated in shake flasks with YSC or YP medium containing 2% (w/v) glucose, glycerol, or ethanol as the sole carbon source or a mixture of the two carbon sources (1:1, 1% v/v each) for 144 h at 30 °C. All data represent the mean ± standard deviation of biological triplicates

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