Fig. 3
From: Bioconversion of vitamin D3 to bioactive calcifediol and calcitriol as high-value compounds
NAD(P)H-regeneration system enhances the bioconversion of VD3 to calcifediol and calcitriol in engineered R. erythropolis. Sufficient reducing equivalents (NADH/NADPH) obtained by the NAD(P)H-regeneration system (GlcDH from B. megaterium) provide more electrons required for the catalysis of the hydroxylation reaction. Moreover, nisin treatment contributes to increased permeability of the cell membrane