Fig. 5
Influence of growth conditions on the production of farnesene and bisabolene by the Synechocystis strains engineered in this study. Cells pre-cultivated under standard photoautotrophic conditions were inoculated at the initial cell density of 106 cells mL−1 (OD750 nm = 0.02) and grown under dodecane (20% vol/vol) at the indicated lights (A) or temperatures (B), prior to measuring bisabolene and farnesene productions. C Cells washed and resuspended in nitrogen-free medium were overlaid with dodecane (20% vol/vol) after 3 days of incubation in growth medium supplemented at the indicated times (green arrows) with the same amounts of nitrogen provided as successive additions of either 4 mM nitrate (NO3) or 2 mM urea (CO(NH2)2). Error bars represent standard deviation from three biological replicates