Fig. 4

Analysis of hydrolysis product formation by exo-arabinofuranosidases A and endo-arabinanases B by TLC. Standard reactions were performed by incubating 5 g L⁻¹ of SBA or DA with 211.83 nM MC57GH51, 19.69 nM MC60GH51, 457.64 nM MC60GH43, 2.64 µM MC68GH43-2, 130.37 nM MC72GH43-2 or 1.22 µM MC68GH43-1 at the enzymes’ optimal temperature and pH for different time periods. 10 µL samples were separated three times on TLC silica plates using chloroform:acetate:water (6:7:1, v/v/v) as a solvent