Fig. 6

Hydrolysis of SBA or DA by different combinations of endo-arabinanases and exo-arabinofuranosidases. SBA or DA (5 g L⁻¹) were incubated in 200 µL reactions containing 25 mM citrate–phosphate buffer pH 5.5 with single enzymes or enzyme combinations using enzyme concentrations of 75 nM (endo-arabinanases MC68GH43-1 and MC72GH43-2) and 150 nM (exo-arabinofuranosidases MC57GH51, MC60GH51, MC60GH43 and MC68GH43-2) at 50 °C for 24 h. A Evaluation of the yield of arabinose (HPAEC-PAD) and reducing sugar (DNS assay with arabinose as standard) released from SBA and DA in mg L⁻¹. B, C HPAEC-PAD analysis of reactions carried out to identify the simplest enzyme combination which can fully hydrolyze SBA