Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases

Table 1 Construction and functional screening of metagenomic libraries to identify degradative enzymes

Name of libraries	Fosmid backbones	Origins	Number of clones	Size of inserts	Positive clones for sequencing
ED library	pCT3FK	Elephant feces	1372	~ 40 kb	3 (2 CMCases^a, 1 xylanase)
BM1T library	pCT3FK	Thermophilic digestate, fermenter	980	~ 40 kb	15 (3 arabinoxylanases, 9 dual activities^b, 3 xylanases)
MOD18 library	pCC1FOS	Thermophilic digestate, fermenter, 50 °C	20000	~ 40 kb	18 (4 arabinanases, 14 CMCases)
T1T2 library	pCC1FOS	Thermophilic digestate, fermenter, 55 °C	5000	~ 40 kb	19 (2 arabinanases, 2 xylanases, 15 CMCases)
Others	pCC1FOS/pCT3FK	various	5424	~ 40 kb	0
Total			32776		55

Positive clones were screened by using chromogenic polysaccharide hydrogel (CPH) substrates and insoluble chromogenic biomass (ICB) substrates
^aCMCases: carboxymethyl cellulases
^bDual activities: both xylanase and arabinoxylanase activities

ISSN: 2731-3654