Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases

Table 3 Statistics of genes for putative fibrolytic enzymes from 55 sequenced fosmids

CAZy family^a	Putative activity ^b	Gene no.
Hemicellulose degrading ability (37 putative CAZymes)
GH10	Endo-1,4-beta-xylanase [EC:3.2.1.8]	13
GH2	β-mannosidase [EC:3.2.1.25]	4
GH11	Endo-1,4-beta-xylanase [EC:3.2.1.8]	4
GH67	α-Glucuronidase [EC:3.2.1.139]	1
CE1	Endo-1,4-beta-xylanase [EC:3.2.1.8]	2
CE4	Endo-1,4-beta-xylanase [EC:3.2.1.8]	3
CE6	para-Nitrobenzyl esterase [EC:3.1.1.-]	1
GH4	α-Galactosidase [EC:3.2.1.22]	1
GH127	α-Galactosidase [EC:3.2.1.22]	1
GH43	Arabinoxylan arabinofuranohydrolase [EC:3.2.1.55]	6
GH16	Endo-1,3–1,4-beta-glycanase ExoK [EC:3.2.1.-]	1
Cellulose degrading ability (9 putative CAZymes)
GH3	β-Glucosidase [EC:3.2.1.21]	3
GH9	Endo-glucanase [EC:3.2.1.4]	3
GH94	Cellobiose phosphorylase [EC:2.4.1.20]	1
GH1	β-Glucosidase [EC:3.2.1.21]	1
GH8	Endo-glucanase [EC:3.2.1.4]	1
Pectic substrate and hemicellulose degrading ability (12 putative CAZymes)
GH43	Endo-1,5-alpha-l-arabinosidase [EC:3.2.1.99]	5
GH51	α-l-Arabinofuranosidase [EC:3.2.1.55]	4
GH43	β-Galactosidase [EC:3.2.1.23]	2
GH2	β-Galactosidase [EC:3.2.1.23]	1
Others
GH24	Lysozyme [EC:3.2.1.17]	1
GH73	N-acetylmuramoyl-l-alanine amidase [EC:3.5.1.28]	1
GH13	Starch synthase (maltosyl-transferring) [EC:2.4.99.16]	2

In total 71 putative CAZyme-encoding genes were annotated by dbCAN database; this table shows the merged results of both dbCAN and KEGG annotations
^aThe glycoside hydrolases/carbohydrate esterase families were predicted by use of the dbCAN database;
^bEnzyme functions were predicted by use of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database

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