From: Progress and perspectives of enzymatic preparation of human milk fat substitutes
Product type | Substrates | Enzyme (dosage) | Yield | Ref. | |
---|---|---|---|---|---|
OPO/OPL | Fractionated palm stearin | OA + LA | NS 40,086 (8%) | sn-OPO, sn-OPL yield: 32.48%, 25.49%; sn-2 PA content: 87.75% | [15] |
Fractionated palm stearin | OA + LA | NS 40,086 (10%) | OPL content: 45.8% | [3] | |
OPL content: 43.7% | |||||
Tripalmitin | FFAs from tea camellia seed oil | Lipozyme RM IM (10%) | sn-2 PA content: 94.51%, sn-1,3 OA content: 69.01% | [26] | |
Fractionated palm stearin | OA + LA | Lipozyme RM IM (8%) | OPL content: 48.37%; sn-2 PA content: 84.70% | [27] | |
HMFS contained PUFAs | Tripalmitin | FFAs from camelina oil | Heterologous Rhizopus oryzae lipase immobilized on Lewatit VP OC 1600 (8%) | HMFS yield: 52% | [16] |
Tripalmitin | FFAs from microalgal oil | Lipozyme RM IM (90Â mg) | sn-2 PA content: 89.0%, sn-1,3 PUFA content: 81.3% | [28] | |
TAGs from Nannochloropsis oculata | FFAs from Isochrysis galbana | Novozym 435/Lipozyme TL IM/Lipozyme 435/Lipozyme RM IM (10%) | PUFA content: 13.92–17.12%; 59.38–68.13% PA at sn-2 position | [18] | |
MLCT-type HMFS | Cinnamomum camphora seed oil | OA | Lipozyme RM IM (12.5%) |  > 70% of MCFAs at sn-2 position, 78.69% OA at sn-1,3 positions | [29] |
Cinnamomum camphora seed oil | OA | Lipozyme RM IM (10%) | OMO yield: 64.45% | [9] | |
Algal oil | lauric acid | Lipozyme AOAB8 (12%) | Lauric acid, DHA content: 30.91%, 44.68% | [24] |