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Table 1 HMFS synthesis through one-step acidolysis

From: Progress and perspectives of enzymatic preparation of human milk fat substitutes

Product type

Substrates

Enzyme (dosage)

Yield

Ref.

OPO/OPL

Fractionated palm stearin

OA + LA

NS 40,086 (8%)

sn-OPO, sn-OPL yield: 32.48%, 25.49%; sn-2 PA content: 87.75%

[15]

Fractionated palm stearin

OA + LA

NS 40,086 (10%)

OPL content: 45.8%

[3]

OPL content: 43.7%

Tripalmitin

FFAs from tea camellia seed oil

Lipozyme RM IM (10%)

sn-2 PA content: 94.51%, sn-1,3 OA content: 69.01%

[26]

Fractionated palm stearin

OA + LA

Lipozyme RM IM (8%)

OPL content: 48.37%; sn-2 PA content: 84.70%

[27]

HMFS contained PUFAs

Tripalmitin

FFAs from camelina oil

Heterologous Rhizopus oryzae lipase immobilized on Lewatit VP OC 1600 (8%)

HMFS yield: 52%

[16]

Tripalmitin

FFAs from microalgal oil

Lipozyme RM IM (90 mg)

sn-2 PA content: 89.0%, sn-1,3 PUFA content: 81.3%

[28]

TAGs from Nannochloropsis oculata

FFAs from Isochrysis galbana

Novozym 435/Lipozyme TL IM/Lipozyme 435/Lipozyme RM IM (10%)

PUFA content: 13.92–17.12%; 59.38–68.13% PA at sn-2 position

[18]

MLCT-type HMFS

Cinnamomum camphora seed oil

OA

Lipozyme RM IM (12.5%)

 > 70% of MCFAs at sn-2 position, 78.69% OA at sn-1,3 positions

[29]

Cinnamomum camphora seed oil

OA

Lipozyme RM IM (10%)

OMO yield: 64.45%

[9]

Algal oil

lauric acid

Lipozyme AOAB8 (12%)

Lauric acid, DHA content: 30.91%, 44.68%

[24]