Table 2 HMFS synthesis through one-step transesterification
From: Progress and perspectives of enzymatic preparation of human milk fat substitutes
Type of acyl donor | Substrates | Enzyme (dosage) | Yield | Ref. | |
|---|---|---|---|---|---|
Fatty acid ester | Tripalmitin | Ethyl oleate | Immobilized Candida parapsilosis Lipase/Acyltransferase (5%) | OA incorporation: 51Â mol%; sn-2 OA content: 15% | [38] |
Palm stearin oil | Ethyl oleate | Lipozyme RM IM (10%) | OPO content: 29.1%; sn-2 PA content: 54.5%, sn-1,3 OA content: 73.8% | [39] | |
Palm stearin | Peanut oil fatty acid ethyl esters | Novozym 40,086 (8%) | OPO content: 46.30%; 60.70% PA at sn-2 position | [40] | |
Tibet yak ghee | Sunflower oil ethyl esters and ethyl oleate | Lipozyme RM IM (10%) | LA content: 17.59%; 56.40% PA at sn-2 position | [30] | |
Oil/fat | Medium-chain triacylglycerols | ARA-rich fungal oil | Lipozyme 435 (8%) | MLCTs content: 53.75% | [10] |
TAGs rich in OPO and OPL | Coconut oil | NS 40,086 (8%) | MLCTs content: 70.44% | [22] | |
Catfish oil | Coconut oil | NS 40,086 (8%) | MLCTs content: 62.14%; relative content of PA at sn-2 position: 46.14% | [23] | |
Lard | Sunflower oil + canola oil + palm kernel oil + palm oil + algal oil + microbial oil | Lipozyme RM IM (11%) | Similarity in total and sn-2 fatty acid, PUFA, TAG composition: 92.5, 90.3, 61.5, 71.9 | [41] | |
Lard | Sunflower oil + canola oil + palm kernel oil + palm oil + algal oil + microbial oil | Lipozyme RM IM (25 g) | Degree of similarity in TAG composition: 72.3; 39.2% PA at sn-2 position; ARA, DHA content: 0.5%, 0.3% | [33] | |