Fig. 4

Analyses of biomass enzymatic saccharification in the PtoMYB115 transgenic lines and WT. a Hexose yields (% biomass) released from enzymatic hydrolysis after the pretreatment with 4% H₂SO₄, 4% NaOH, or 10% CaO pretreatments. b Bioethanol yields (% biomass) obtained from yeast fermentation using total hexose contents released from enzymatic hydrolysis after pretreatments. c SEM images of in situ enzymatic digestion of stem cross sections after 4% NaOH pretreatment and sequential enzymatic hydrolysis, scale bar is 100 μm or 5 μm; d SEM images of biomass residue obtained from 4% NaOH pretreatment and sequential enzymatic hydrolysis. Allows as rough point. Scale bar is 5 μm. Data represent mean ± SD of three technical replicates. Student’s t-test between transgenic line and WT as **P < 0.01