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Fig. 1 | Biotechnology for Biofuels and Bioproducts

Fig. 1

From: Characterization of an Entner–Doudoroff pathway-activated Escherichia coli

Fig. 1

Comparison of cell growth on glucose among EMPP-disrupted strains and the effect of mutations on cell growth of the evolved strains and their background strains. a Growth rate profiles (OD600) were compared for three groups of E. coli strains: wild type with MG1655, complete disruption of EMPP with background strain (∆pfkAB), and evolved strain (ALE-1), and partial disruption of EMPP with the phosphofructokinase I step (∆pfkA) and phosphoglucose isomerase step (∆pgi). Error bars indicate standard deviations of measurements for three independent biological replicates. b Genetic characterization of five independent evolved ∆pfkAB isolate strains with genome resequencing analysis. The number of unique mutations per gene is noted within brackets. c The effect of mutations on cell growth were determined by introducing single or multiple mutations in the background strain ∆pfkAB. For the mutation in ptsG, the IS5 insertion disrupted the protein, and consequently, PtsG would lose its functionality. Thus, we knocked out the ptsG gene instead of knocking-in the IS element. Error bars indicate standard deviations of growth measurements for three independent biological replicates

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