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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Characterization of an Entner–Doudoroff pathway-activated Escherichia coli

Fig. 2

Functional analysis of major mutations found in the evolved strains. a RNA-seq transcriptome analysis illustrated the expression level of genes involved in glucose uptake system, glycolytic pathway, TCA cycle and transcriptional regulons including gntR and galR, crp regulons and sugar phosphate stress. Expression change (log2 fold change) of pfkA and gnd genes was unable to be calculated due to loss of expression and it was marked with an asterisk (*). Unexpected expression level of the pfkB gene of ALE-1 was affected by a remained coding sequence of pfkB (Additional file 1: Figure S8). b Functional validation experiments identified effective target genes among mutated genes from ALE-1: lacking the Gnd function, and loss of function for GntR and de-repression of its target genes, edd and eda. Error bars indicate standard deviations of growth measurements for three independent biological replicates. c Combined fluorescence histograms of MG1655, ALE-1, MG1655 Δpgi ΔgntR strains carrying pHexR-GFP. d The schematic diagram illustrates altered glucose metabolism in ALE-1 through the EDP, via enzymes encoded by edd and eda. Transcriptional repression by GntR is indicated with red marks

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