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Table 1 List of mutations identified in the evolved strain pfk_ALE-1

From: Characterization of an Entner–Doudoroff pathway-activated Escherichia coli

Gene

Gene product and its size (number of aa)a

Type of mutationb

Changec in protein and nucleotide

gntR

Gluconate repressor protein (331)

SNP

A141E (GCG → GAG)

galR

Galactose repressor protein (343)

SNP

G271D (GGC → GAC)

ptsG

Glucose-specific PTS enzyme IIBC (477)

INS

IS5 insertion (IS5( +) + 4 bp, coding 815–818/1434 nt)

ugd

UDP-glucose 6-dehydrogenase (388)

DEL

Truncated protein (∆176 bp, coding 992–1167/1167 nt)

gnd

6-Phosphogluconate dehydrogenase (468)

Loss of function (∆1407 bp, cording 1–1407/1407 nt)

wbbL-2

Interrupted rhamnosyltransferase (pseudogene)

Loss of function (∆349 bp, coding 1–349/349 nt)

crp

cAMP receptor protein (210)

SNP

A85S (GCA → TCA)

talB

Transaldolase B (317)

SNP

A247V (GCA → GTA)

  1. aaa, amino acid, UDP uridine 5′-diphosphoglucose, cAMP cyclic adenosine monophosphate
  2. bSNP single nucleotide polymorphism, INS insertion, DEL deletion
  3. c + , insertion; bp base pairs, nt nucleotide, ∆ deletion