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Table 1 Fatty acid composition (mol%) of transgenic seeds overexpressing tandem EhDH domains

From: Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana

FA species

WT

DH-T2-4

DH-T2-5

DH-T2-11

16:0

12.05 ± 0.83A

7.66 ± 0.31C

7.33 ± 0.27C

8.28 ± 0.31B

16:1–9

1.66 ± 0.20A

0.33 ± 0.03B

0.54 ± 0.15B

0.37 ± 0.01B

18:0

10.73 ± 1.32A

4.26 ± 0.28B

4.59 ± 0.37B

4.33 ± 0.24B

18:1–9

11.19 ± 0.82C

15.67 ± 0.49A

14.46 ± 0.30B

14.6 ± 0.44B

18:1–11

2.26 ± 0.23A

1.3 ± 0.08B

1.24 ± 0.05B

1.33 ± 0.03B

18:2

20.81 ± 0.74C

24.95 ± 0.44AB

25.54 ± 0.35A

24.01 ± 0.53B

18:3

14.19 ± 1.23C

16.78 ± 0.59B

17.1 ± 0.37B

18.63 ± 0.46A

20:1–11

14.2 ± 0.54B

20.93 ± 0.14A

20.4 ± 0.47A

20.54 ± 0.70A

Others

12.91 ± 1.12

8.12 ± 0.15

8.8 ± 0.21

7.92 ± 0.14

C16–18

72.89 ± 0.77

70.95 ± 0.22

70.80 ± 0.63

71.54 ± 0.73

SFA

32.06 ± 1.21A

15.31 ± 0.65B

15.84 ± 0.82B

15.90 ± 0.69B

UFA

67.94 ± 1.73B

84.69 ± 0.65A

84.16 ± 0.82A

84.10 ± 0.69A

UFA/SFA

2.12

5.53

5.31

5.29

  1. FA fatty acid, WT wild type, OE overexpression lines, SFA saturated fatty acid, UFA unsaturated fatty acid, UFA/SFA ratio of unsaturated/saturated fatty acids.
  2. Values are reported as the means ± standard deviation of 10 independent biological replicates. The means with the same letters are not statistically significantly different. The statistical analysis of the results was conducted using one-way analysis of variance (P < 0.05)