Fig. 6

Transcriptional intergenic links in the xua cluster The xua cluster is shown at the top (star corresponds to xuaD’). Just below, the black lines correspond to the PCR amplifications. PCR amplifications were performed on genomic DNA (gDNA, upper electrophoresis gel), cDNA (middle electrophoresis gel) obtained after reverse transcription from an arabinoxylan (WAXY-I) grown culture, or mRNA (lower electrophoresis gel). The primers used to study the transcriptional links between two successive genes were localized at the end of the first gene for the direct primer and at the beginning of the second gene for the reverse primer. The amplicons were obtained using the following primer pairs: 1 (1249-E-RT-up/qRT-xuaS-708-rev); 2 (qPCR-xuaS-431-dir/1251-S-RT-do); 3 (1250-E-RT-up and 1252-S-RT-do); 4 (qPCR-xuaR-277-dir/qRT-xuaA-525-rev); 5 (qPCR-xuaA-2nd-378-dir/qPCR-xuaB-584-rev); 6 (1252-E-RT-up-2nd/1254-S-RT-do-3rd); 7 (1253-E-RT-up/1255-S-RT-do); 8 (1254-E-RT-up/1256-S-RT-do); 9 (1252-E-RT-up-2nd/1255-S-RT-do); 10 (1255-E-RT-up-2nd/1257-S-RT-do); 11 (1256-E-RT-up/1258-S-RT-do); 12 (1257-E-RT-up/1259-S-RT-do); 13 (1258-E-RT-up/1260-S-RT-do); 14 (1259-E-RT-up/1261-S-RT-do); 15 (1260-E-RT-up/1262-S-RT-do)