Table 2 Catalytic parameters of XuaD, E, F, G, H and J
XuaD | XuaE | XuaF | XuaG | XuaH | XuaJ | ||
|---|---|---|---|---|---|---|---|
AXOS | A3X | IU: 2381.7 ± 6.7 | NA | NA | NA | ND | ND |
Products: Ara, X2 | |||||||
A2XX | IU: 1263.0 ± 46.1 | NA | IU: 202.3 ± 18.7 | NA | |||
Km 1.4 ± 0.3 | Products: Xyl, A2X? | ||||||
kcat 3408.0 ± 356.6 | |||||||
kcat/Km 2411.9 ± 303.3 | |||||||
Products: Ara, X3 | |||||||
A2,3XX | IU: 1256.3 ± 50.2 | IU: 1938.0 ± 109.6 | ND | NA | |||
Km 1.8 ± 0.2 | Km 0.1 ± 0.003 | ||||||
kcat 4191.3 ± 117.3 | kcat 2253.5 ± 142.1 | ||||||
kcat/Km 2388.2 ± 182.9 | kcat/Km 23137.8 ± 1355.0 | ||||||
Products: Ara, X3 | Products: Ara, A2XX, A3XX? | ||||||
XA3XX | IU: 1218.9 ± 38.5 | IU: 1.6 ± 0.05 | IU: 166.7 ± 12.8 | IU: 21.2 ± 0.2 | |||
Products: Ara, X4 | Products: Ara, X4 | Products: Xyl, XA3X ? | Products: Xyl, A3XX?a | ||||
XAXXMIX | IU: 1325.8 ± 7.8 | NA | ND | IU: 18.0 ± 0.3 | |||
Products: Ara, X4 | Products: Xyl, A2XX, A3XX ?a | ||||||
XA2,3XX | IU: 1.5 ± 0.03 | IU: 1927.2 ± 100.4 | IU: 29.7 ± 2.8 | IU: 20.3 ± 0.2 | NA | NA | |
Products: Ara, X4 | Products: Ara, XA2XX, XA3XX | Products: Xyl, XA2,3X ? | Products: Xyl, A2,3XXa | ||||
XOS | X5 | ND | ND | IU: 25.3 ± 0.7 | IU: 6.2 ± 0.5 | ND | ND |
Products: Xyl, X4, X3 | Products: Xyl, X4, X3, X2a | ||||||
X4 | ND | ND | IU: 87.2 ± 7.6 | IU: 23.3 ± 0.5 | |||
Products: Xyl, X3, X2 | Products: Xyl, X3, X2a | ||||||
X3 | ND | ND | IU: 158.7 ± 21.1 | IU: 24.5 ± 1.6 | |||
Products: Xyl, X2 | Products: Xyl, X2a | ||||||
X2 | ND | ND | NA | NA | |||
1-Naphthyl acetate | ND | ND | ND | ND | IU: 3504.6 ± 18.9 | IU: 21130.4 ± 676.4 | |
Km: 3.5 ± 1.1 | Km: 0.2 ± 0.02 | ||||||
kcat: 5435.7 ± 846.4 | kcat: 21380.0 ± 440.0 | ||||||
kcat/Km: 1628.1 ± 295.7 | kcat/Km: 88703.9 ± 7849.6 |