Skip to main content
Fig. 9 | Biotechnology for Biofuels and Bioproducts

Fig. 9

From: Precursor prioritization for p-cymene production through synergistic integration of biology and chemistry

Fig. 9

Production in fermentation media. A 1,8-cineole production comparison from strains with (DM07 and DM08) and without (DM02 and DM04) the acetate pathways deleted from different glucose concentrations; experiments were run in test tubes using 8 mL of the fermentation medium. B 1,8-cineole production time course; 50 mL cultures were grown in shake flasks using the fermentation media with 1.5% glucose. Cultures were inoculated to an OD600nm of 0.1 from an overnight culture, grown at 37 °C and induced at an OD600nm of 1.2 with 1 mM isopropyl β-D-1-thiogalactopyranoside, at the time of induction a 10% dodecane overlay was added and the temperature was changed to 30 °C. All experiments were run in triplicates and the error bars represent the standard deviation

Back to article page