Fig. 1

Characterization of BnCPC proteins. A Phylogenetic relationship of BnCPC and other known MYB repressors in plants. BnCPC homologues were highlighted with the black rhombi. Alignment of full amino acid sequences of MYB repressors was done by MUSCLE software, and phylogenetic tree was constructed using the neighbor-joining method by MEGA software 7.0. Numbers at the nodes indicated the reliability percentage of bootstrap values based on 1000 replications. B Alignment and structural domains of BnCPC and MYB repressors. Protein sequences were aligned using ClustalX. The R2 and R3 domains were indicated with grayish white and gray boxes, respectively. Three α-helices of both R2 and R3 domains were indicated in boxes. The conserved MYB–bHLH interaction motif ([D/E]Lx2[R/K]x3Lx6Lx3R) of R3 domain was underlined with a black bar. The conserved motifs in the C-terminus were highlighted with boxes and numbers. C1 domain, LIsrGIDPxT/SHRxI/L; C2 domain (the EAR repression motif), pdLNLD/ELxiG/S; C3 domain (zinc-finger motif), Cx2Cx9CxC; C4 domain, GY/FDFLGL motif; C5 domain, TLLLFR repressor motif. The following GenBank or Arabidopsis TAIR accession numbers were used, PpMYB18 (KT159234.1), VvMYBC2-L1 (JX050227), VvMYBC2-L2 (ACX50288), VvMYBC2-L3 (KM046932), FaMYB1 (AF401220), MtMYB2 (XM_003616340), PtrMYB182 (KP723392), AtMYB4 (AF062860), PhMYB27 (AHX24372), MdMYB16 (HM122617), AtMYBL2 (AEE35154), IlMYBL1 (ASR83103.1), LhR3MYB1 (BBG71951.1), LhR3MYB2 (BBG71953.1), AtCPC (AT2G46410.1), PhMYBx (AHX24371.1), MlROI1 (AGC66791.1), SlMYBATV (NP_001352307), FhMYBx (MT210095), MaMYBx (QJH86892.1)