Fig. 3
BnCPC repressed anthocyanin accumulation in B. napus. A Phenotype of J9712 and BnCPC overexpression (OE-CPC) lines under low temperature (10 °C) and normal temperature (23 °C). Scale bar represented 1 cm. B Anthocyanin content in extracts from seedlings in A. (A530-0.25 × A657)/gram fresh weight was considered as the relative anthocyanin content. Three biological replicates were performed, and 10 plants were pooled as one replicate. FW, fresh weight. Values represented the mean ± SD (n = 3). Different letters represented statistically significant differences (two-way ANOVA, p < 0.05). C The expression level of BnDFR, BnLDOX, and BnUF3GT in rapeseed seedlings from A. Expression levels were normalized to B. napus Actin-7 (NC_027775.2), and the expression level of J9712 under normal temperature was set at 1. Values represented the mean ± SD (n = 3)