Fig. 1

Identification of sugar transporters related to cellulase production. A DEGs of sugar transporters in T. reesei Rut-C30 grown on cellulose as compared to glucose, which are predicted to transport a maltose, b fucose, c xylose, d lactose, e d-galactonate, and f unknown sugars. The red pentagrams mark the genes studied in this research, including M419DRAFT_127980 (lac1), M419DRAFT_109243 (crt1), M419DRAFT_138519 (gst), M419DRAFT_137795 (mfs). Each gene was presented using its gene ID from the T. reesei Rut-C30 genome database (https://www.ncbi.nlm.nih.gov/genome/323?genome_assembly_id=49799). B Transcription levels of sugar transporter genes crt1, mfs, gst, and lac1 in T. reesei Rut-C30 cultured on TMM + 2% cellulose/glucose at 24 h, 72 h, 120 h, and 168 h. C Transmembrane domains of four sugar transporters obtained from the webserver Protter. The green square represents n-glycosylation motif and red circle represents the signal peptide. D The (hemi)cellulase activities and protein secretion of T. reesei Ku70, ΔMFS, ΔGST, and ΔLAC1 cultured in TMM + 2% cellulose for 120 h. E The colony diameters and F spore amounts of T. reesei Ku70, ΔMFS, ΔGST, and ΔLAC1 grown on TMM plates with 2% cellulose at 120 h. Values are the mean of three biological replicates and error bars are the standard deviation of these three replicates. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001) as assessed by Student’s t test