Fig. 2

The effect of the three sugar transporters on sugar transport and biomass accumulation. A HPLC analysis of residual sugars in the supernatant of T. reesei Ku70, ΔMFS, ΔGST, and ΔLAC1 cultured on 1% cellobiose + 50 μg/mL 1-deoxynojirimycin, 1% lactose, 1% glucose, 1% galactose, or 1% mannose. B Growth of T. reesei Ku70, ΔMFS, ΔGST, and ΔLAC1 on different sugars for 168 h. C The corresponding colony diameters in (B) were calculated. Values are the mean of three biological replicates and error bars are the standard deviation of these three replicates. D Growth analysis of S. cerevisiae 303-gh1-pRS426, 303-gh1-MFS, 303-gh1-GST, and 303-gh1-LAC1 in the presence of 1% cellobiose and S. cerevisiae 4000-pRS426, 4000-MFS, 4000-GST, and 4000-LAC1 in the presence of 2% maltose, 1% lactose, 1% glucose, 1% galactose, or 1% mannose. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001) as assessed by Student’s t test