Fig. 1

Enzymatic characterization of exo-1,3-β-glucanase G9376. (a) pH- and (b) temperature-dependent activity of exo-1,3-β-glucanase G9376 toward 0.2% (w/v) laminarin as determined by the reducing sugar assay. (c) Residual activity of exo-1,3-β-glucanase G9376 toward 0.2% (w/v) laminarin after 1 h-incubation at different temperatures as determined by the reducing sugar assay. (d) Specific activity expressed as Units (µmol reducing end/min, µmol Glc/min) per mg of exo-1,3-β-glucanase G9376 toward LAM5ol. The amounts of reducing ends and Glc were determined by the reducing sugar and GO-POD assays, respectively. Values are mean ± SD, n = 3. Analyses shown in (b–d) were performed at pH 5.0. [LAM5ol, 1,3-β-D-laminaripentaitol borohydride; Glc, D-Glucose]