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Table 2 Determination of degradation products obtained from a ΔG7048-pretreated LAM5 upon incubation with exo-1,3-β-glucanase G9376

From: Characterization of two 1,3-β-glucan-modifying enzymes from Penicillium sumatraense reveals new insights into 1,3-β-glucan metabolism of fungal saprotrophs

 

µg sugars

nmol sugars

ΔG7048-pretreated LAM5

LAM5 + G9376

ΔG7048-pretreated LAM5 + G9376

ΔG7048-pretreated LAM5

LAM5 + G9376

ΔG7048-pretreated LAM5 + G9376

Glc

6.2 ± 0.1

3.4 ± 0.1

34.3 ± 0.3

18.9 ± 0.5

LAM2

2.5 ± 0.1

4 ± 0.1

4.1 ± 0.1

7.4 ± 0.2

11.8 ± 0.1

12.1 ± 0.1

Degradation by-product X

2.7 ± 0.2

5.3 ± 0.4

Hybrid oligosaccharides

7.7 ± 0.1

 

5.9 ± 0.05

    

Glc:LAM2

2.9

1.5

  1. Amounts of degradation products (expressed as µg and nmol sugars) obtained from a ΔG7048-pretreated LAM5 (ΔG7048-pretreated LAM5 + G9376) and LAM5 (LAM5 + G9376) upon 1 h-incubation with exo-1,3-β-glucanase G9376 as determined by HPLC analysis. The amounts of products from a ΔG7048-pretreated LAM5 (ΔG7048-pretreated LAM5) are reported as control. Values are mean ± s.d. (n = 3). — not detected. [Glc D-Glucose LAM2 laminaribiose]