Fig. 5

Light-induced NAD(P)H fluorescence kinetics in Syn::P_cpcBYqjM. Reaction conditions: D-Glu = 2.5 mM, 1a = 10 mM (added to the sample 30 min prior measurement). Cells (chla = 2.5 µg mL⁻¹) were placed in dark for 10 s (black bar) followed by illumination under red actinic light (200 µmol m⁻² s⁻¹, red bar) for 180 s and finally in dark for 60 s (black bar). See Materials and Methods section for the detailed procedure