Table 1 Yields (YP/S) and titers of engineered C. glutamicum strains cultivated as 1Â mL cultures
From: Metabolic engineering of Corynebacterium glutamicum for acetate-based itaconic acid production
Strain | Genotype | YP/S, mmol mol−1 | Titer, g L−1 |
|---|---|---|---|
ITA1 | (pEKEx2-malEcadopt) | 8 ± 2 | 0.32 ± 0.09 |
ITA2 | IDHA94D (pEKEx2-malEcadopt) | 9 ± 2 | 0.35 ± 0.06 |
ITA3 | IDHG407S (pEKEx2-malEcadopt) | 13 ± 6 | 0.49 ± 0.18 |
ITA4 | IDHR453C (pEKEx2-malEcadopt) | 20 ± 5 | 0.75 ± 0.18 |
ITA5 | IDHR453C Ppck::PdapA-A8 (pEKEx2-malEcadopt) | 10 ± 1 | 0.39 ± 0.00 |
ITA6 | IDHR453C Ppck::PdapA-A16 (pEKEx2-malEcadopt) | 19 ± 4 | 0.73 ± 0.13 |
ITA7 | IDHR453C Ppck::PdapA-C5 (pEKEx2-malEcadopt) | No growth | |
ITA8 | IDHR453C Ppck::PdapA-C7 (pEKEx2-malEcadopt) | 12 ± 1 | 0.39 ± 0.00 |
ITA9 | ΔramB (pEKEx2-malEcadopt) | 36 ± 4 | 1.54 ± 0.18 |
ITA10 | ΔramB (pEKEx2-malEcadopt-ramA) | 39 ± 6 | 1.35 ± 0.23 |
ITA11 | ΔramB ΔglnE (pEKEx2-malEcadopt) | 18 ± 4 | 0.60 ± 0.21 |
ITA12 | ΔramB Δgdh (pEKEx2-malEcadopt) | 62 ± 12 | 2.36 ± 0.75 |
ITA13 | ΔramB Δgdh IDHR453C (pEKEx2-malEcadopt) | 81 ± 9 | 3.43 ± 0.59 |