Fig. 4
From: Transformation and gene editing in the bioenergy grass Miscanthus
Sanger sequencing of lw1 in transgenic edited and isogenic wild-type Miscanthus plants. A sequence alignment of the highly conserved region targeted by gRNAs lw1G1 (top panel), lw1G2 (middle panel) and lw1G3 (bottom panel), from edited and wild-type Msa S1, Mxg Freedom, and Msi UI1. The gRNA is shown as a blue arrow above the alignment and deleted bases are marked with a hyphen (–). For the sake of simplicity, only one representative sequence of each type of deletion is shown. A complete alignment is available as aligned fasta files (Additional file 4)