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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Spatial–temporal regulation of fatty alcohol biosynthesis in yeast

Fig. 2

Coordinated fatty alcohol biosynthesis by optimizing a fatty acyl-CoA derived pathway. A Scheme of fatty alcohol biosynthetic pathways derived from fatty acids (CAR pathway, blue arrow) and acyl-CoA (FaCoAR or FAR pathway, orange arrow). B Partial coordination of fatty alcohol biosynthesis with peroxisome biogenesis and precursor supply promoted fatty alcohol production. Fatty alcohol titer of peroxisomal fatty alcohol biosynthetic pathway driven by constitutive promoters (PTDH3, PTEF1) was relatively low. Consistently, using GAL promoters of galactose catabolism (with deletion of GAL80) to drive the peroxisomal biosynthetic pathway significantly improved fatty alcohol production by 3.25 folds. FA, fatty acids; FOH, fatty alcohols. C Overexpression of peroxins PEX7 and PEX28 promoted fatty alcohol production of peroxisomal CAR pathway (per-CAR). D Residual free fatty acids of fatty alcohol producing strains with per-CAR pathway. E Comparison of fatty alcohol titer between FAR pathway and CAR pathway in peroxisomes. GNFOH132, the strain GN15 with an empty expression vector ; per-FAR, fatty alcohol production via peroxisomal FAR pathway. per-CAR, fatty alcohol production via peroxisomal CAR pathway. Data are presented as mean ± SD of three or four biologically independent samples with displayed data points. Red asterisks indicate statistical significance as determined using paired t test (*P < 0.05; *** < 0.001)

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