Fig. 3

Molecular structure of Mol3 laccase. Cartoon representation of the 3D model structure of Mol3 laccase built with PyMol using 6H5Y.PDB as the template. Residues forming the first sphere of T1 catalytic copper are depicted as yellow sticks; residues mutated during the directed evolution of laccase towards alkaliphilicity are depicted as pink sticks; residues of difference between 7A12 and RY2 starting laccases are shown as blue sticks, and residues found to confer thermal stability to laccase in the bottom-up designing strategy are shown as orange sticks. Blue spheres represent the four catalytic coppers