Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing

Table 4 Production and thermal tolerance of C-le B and Mol2 mutated variants

Laccase	Mutation	Activity (U/L)	T₅₀ (10 min) °C
C-LeB	–	1812	74 ± 0.1
Mol2	–	1294	78 ± 0.1
Top down
Mol2R-424	T424V	753	76 ± 0.07
Mol2R-426	S426N	1231	78 ± 0.04
Mol2R-429	T429S	531	76 ± 0.07
Mol2R-430	D430P	1522	78 ± 0.06
Mol2R-439	T439R	673	78 ± 0.08
Mol2R-441	N441D	1891	74 ± 0.09
Bottom up
C-LeB-424	V424T	1500	72 ± 0.1
C-LeB-429	S429T	2791	75 ± 0.1
C-LeB-441	D441N	581	78 ± 0.1
C-LeB-DM1	V424T, S429T	1897	77 ± 0.09
C-LeB-DM2	V424T, D441N	1763	75 ± 0.04
C-LeB-DM3	S429T, D441N	1927	74 ± 0.1
Mol3	V424T, S429T, D441N	1180	80 ± 0.06

Laccase activities detected in the liquid extracts of S. cerevisiae clones cultured in flasks and T₅₀ (10 min) values of C-leB and Mol2 laccases and their mutated variants (determined with ABTS, pH 3)

ISSN: 2731-3654