Fig. 6

Evaluation of ferulic acid assimilation and catabolism by R. fluviale LM-2. A Cell culture in minimal medium (YNB) containing 0.1% glucose with/without 1.25 mM ferulic acid. Control: minimal medium (YNB) + 0.1% glucose + 1.25 mM ferulic acid without cells; B detection of ferulic acid by capillary electrophoresis (UV-214 nm) at different time points (6 h, 12 h and 24 h). Comparison between the culture containing ferulic acid and R. fluviale LM-2 cells and the control (without cells). C Intracellular metabolite identification after 12 h of cultivation. Comparison between the culture of R. fluviale LM-2 cells with/without ferulic acid in the medium. (Ferulic acid: MW 194 g/mol; C₁₀H₁₀O₄; retention time 5.6 min), (4-vinyl-guaiacol: MW 150 g/mol; C₉H₁₀O₂; retention time 4.7 min and 6.5) and (vanillin: MW 152 g/mol; C₈H₈O₃; retention time 4.7 min)