Fig. 2
HPAEC–PAD chromatograms showing soluble products generated upon incubation of TtLPMO9E with PASC, or a combination of PASC and differently substituted WAX at pH 6.5 and 40 °C, for 16 h. All reactions contained gallic acid (GA) as reductant. Oxidized cello-oligosaccharides resulting from PASC degradation are labelled as “GlcXox”, where X indicates the degree of polymerization. Regions that show products resulting from LPMO-catalyzed degradation of WAX22 are indicated by grey arrows and brackets. The reactions were carried out in triplicate and representative chromatograms are shown. No reaction products were detected in control reactions lacking reductant or the LPMO (Additional file 1: Fig. S1). Native cello-oligomers, which are present in small amounts, were not identified; they elute in the 2–11 min area and small non-annotated peaks likely belonging to such native products are visible in the 5–11 min sections of the chromatograms