Fig. 3

HPAEC–PAD chromatograms showing soluble products generated upon incubation of a mixture of PASC and WAX38 (a) or WAX22 (b, c) with TtLPMO9E and/or UmGH62 at pH 6.5 and 40 °C, for 16 h. Panel c shows the effect of lowering the dose of UmGH62 from 15 µM to 1.5 µM. Oxidized cello-oligosaccharides resulting from PASC degradation are labelled as “GlcX_ox”, where X indicates the degree of polymerization. Regions that show novel products resulting from LPMO-catalyzed degradation of WAX22 or WAX38 in the presence of UmGH62 are indicated by grey arrows and brackets. Control reactions showed that product formation depends on the presence of both the LPMO and the reductant (i.e., a catalytically active LPMO; see panels a–c and on the presence of cellulose (Additional file 1: Fig. S3, which shows reactions with WAX alone). Control reactions without LPMO contained 0.5 mM CuSO₄, to account for possible (copper-catalyzed) side reactions