Fig. 4

HPAEC–PAD chromatograms showing soluble products generated upon incubation of TtLPMO9E or TtLPMO9G with mixtures of PASC and SpAGX (a, b) or BeGX (c), in the presence or absence of debranching enzymes. Oxidized cello-oligosaccharides resulting from PASC degradation are labelled as “GlcX_ox”, where X indicates the degree of polymerization. Regions that show novel products resulting from LPMO-catalyzed degradation of SpAGX or BeGX in the presence of the debranching enzymes are indicated by grey arrows and brackets. All reactions were carried out at pH 6.5 and 40 °C, for 16 h. Chromatograms for control reactions without reductant or LPMO did not show oxidized products (Additional file 1: Figs. S4 and S5)