Fig. 10

The analysis of metabolites in 32 DAF seeds of MSTRG.22563 transgenic plants. A The mass signal of metabolites related to glycolysis, including G6P, F6P, GAP&DHAP, 2PGA&3PGA, PEP, PYR, and G3P. Values are means ± SD (n = 4–8). B The mass signal of metabolites involved in respiration and TCA cycle, including 2-P-glycerate, glycolate, glyoxylate, glycine, malate, succinate, and fumarate. Values are means ± SD (n = 4–8). C The mass signal of metabolites related to energy metabolism, including ATP, NADH, NAD, and the ratio of NADH/NAD. Values are means ± SD (n = 4–8). * and ** denote significance at P < 0.05 and P < 0.01, respectively, compared with WT based on the Student’s t-test. G6P, Glucose-6-phosphate; F6P, Fructose-6-phosphate; DHAP, dihydroxy-acetone-phosphate; GAP, glyceraldehyde-3-phosphate; G3P, glycerol-3-phosphate; 2PGA, 2-Phosphoglycerate; 3PGA, 3-Phosphoglycerate; PEP, Phosphoenolpyruvate; Pyr, pyruvate. TCA, tricarboxylic acid