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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Regulation of seed oil accumulation by lncRNAs in Brassica napus

Fig. 5

Overexpression of MSTRG.86004 influenced seed oil content of B. napus. A MSTRG.86004 transcript levels in 32 DAF seeds were detected by real-time PCR. Values are means ± SD (n = 3). B and C, Oil content and protein content of mature seeds were analyzed using NIR instruments. Values are means ± SD (n = 8–12). D The 1000 seed weight. Values are means ± SD (n = 8–12). E, F and G, The content of PC, PE, PS, DAG, and TAG were analyzed by LC–MS/MS. Values are means ± SD (n = 8–12). H The content of different TAG species. Values are means ± SD (n = 8–12). I The composition of fatty acid in mature seeds was analyzed by GC-FID. Values are means SD (n = 8–12). * and ** denote significance at P < 0.05 and P < 0.01, respectively, compared with WT based on the student’s t-test. PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; DAG, diacylglycerol; TAG, triacylglycerol

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