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Fig. 1 | Biotechnology for Biofuels and Bioproducts

Fig. 1

From: Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

Fig. 1

Plasmid restriction analysis of Acetobacterium wieringae strain JM transformants. Digestion of pMTL82151, pMTL83151, pMTL84151, and pMTL85151, using NcoI-HF and SpeI-HF, EcoRI-HF, NcoI-HF, NheI-HF, and NotI-HF, respectively, resolved on a 1% agarose gel. Digestion reactions contained 0.5 μg of plasmid DNA and 1 μL of restriction enzymes in a total volume of 50 μL. For comparison, plasmids are shown undigested and digested. Expected band sizes are: 3114 and 2140 bp, pMTL82151; 3475 and 1001 bp, pMTL83151; 3606 and 2697 bp, pMTL84151; 3453 and 276 bp, pMTL85151

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