Table 4 Bacterial strains and plasmids used in this work
Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
Strains | ||
Acetobacterium wieringae strain JM | Wild type | Lab stock |
Acetobacterium wieringae DSM 1911 T | Wild type | DSMZ collection |
Acetobacterium woodii DSM 1030 T | Wild type | DSMZ collection |
Clostridium acetobutylicum DSM 792 T | Wild type | DSMZ collection |
Escherichia coli DH5α | fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80 ∆(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NZYTech |
Escherichia coli TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
Escherichia coli CA434 | E. coli HB101 containing plasmid R702 thi-1 hsdS20 (r-B, m-B) supE44 recAB ara-14 leuB5proA2 lacY1galK rpsL20 (strR) xyl-5 mtl-1 | [28] |
Plasmids | ||
R702 | Tra+, Mob+ conjugative plasmid | [28] |
pMTL82151 | Gram+: pBP1, catP; Gram−: ColE1, catP; application: MCS | [28] |
pMTL83151 | Gram+: pCB102; catP; Gram−: ColE1, catP; application: MCS | [28] |
pMTL84151 | Gram+: pCD6; catP; Gram−: ColE1, catP; application: MCS | [28] |
pMTL85151 | Gram+: pIM13, catP; Gram−: ColE1, catP; application: MCS | [28] |
pMTL83_APO | pMTL83151, PthlA, thlA, ctfAB, adc from C. acetobutylicum | This study |