Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

Table 5 Primers used in this study

Primer^a	Sequence (5´- 3´)^a	Description
SalI_PthlA_fw	GCCGACGCGTCGACATATTGATAAAAATAATAATAGTGGG	Amplification of P_thlA_thlA
BamHI_thlA_rev	CGGGATCCCTAGCACTTTTCTAGCAATATTGC	Amplification of P_thlA_thlA
BamHI_ctfA_fw	CGACCGGGATCCATGAACTCTAAAATAATTAG	Amplification of ctfAB
KpnI_ctfB_rev	GGGGTACCCTAAACAGCCATGGGTCTAAG	Amplification of ctfAB
KpnI_adc_fw	GAGGGGTACCATGTTAAAGGATGAAGTAATTAAAC	Amplification of adc
NotI_adc_rev	GGCCGCGGCCGCTTACTTAAGATAATCATATATAAC	Amplification of adc
pCB102_mid_rev	CTGTTATGCCTTTTGACTATCAC	Plasmid verification of constructs
M13_rev	CAGGAAACAGCTATGAC	Plasmid verification of constructs

ISSN: 2731-3654