Strains or plasmids | Description of genotype | Source |
---|---|---|
Strains | ||
E. coli Trans T1 | F¯φ80(lacZ)ΔM15ΔlacX74 hsdR(rK−, mK+) ΔrecA 1398 endA1 tonA | TransGen |
E. coli Top10 | F¯mcrA Δ (mrr-hsd RMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA araΔ139(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG | TransGen |
B. methylotrophicum | Wild type | ATCC |
B. methylotrophicum/pXY1-NikABCDE | B. methylotrophicum overexpressing the operon of NikABCDE under the control of Pthl | This study |
B. methylotrophicum/pXY1-FhuBCD | B. methylotrophicum overexpressing the operon of FhuBCD under the control of Pthl | This study |
B. methylotrophicum/pXY1-LysA | B. methylotrophicum overexpressing the gene of LysA under the control of Pthl | This study |
B. methylotrophicum/pXY1-DapAB | B. methylotrophicum overexpressing the gene of DapA and DapB under the control of Pthl | This study |
Plasmids | ||
pMCljS | pACYC184, methyltransferase gene of C. ljungdahlii (CLJU c03310), SpeR | [16] |
pXY1 | pCB102 ORI, ColE1 origin; AmpR, EmR | [16] |
pXY1-Pthl-NikABCDE | Derived from pXY1, with the operon of nikA, nikB, nikC, nikD and nikE overexpression | This study |
pXY1-Pthl-FhuBCD | Derived from pXY1, with the operon of FhuB, FhuC and FhuD overexpression | This study |
pXY1-LysA | Derived from pXY1, with the gene of LysA overexpression | This study |
pXY1-DapAB | Derived from pXY1, with the gene of DapA and DapB overexpression | This study |