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Table 1 Strains and plasmids used in this study

From: Increasing lysine level improved methanol assimilation toward butyric acid production in Butyribacterium methylotrophicum

Strains or plasmids

Description of genotype

Source

Strains

E. coli Trans T1

F¯φ80(lacZ)ΔM15ΔlacX74 hsdR(rK−, mK+) ΔrecA 1398 endA1 tonA

TransGen

E. coli Top10

F¯mcrA Δ (mrr-hsd RMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA araΔ139(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG

TransGen

B. methylotrophicum

Wild type

ATCC

B. methylotrophicum/pXY1-NikABCDE

B. methylotrophicum overexpressing the operon of NikABCDE under the control of Pthl

This study

B. methylotrophicum/pXY1-FhuBCD

B. methylotrophicum overexpressing the operon of FhuBCD under the control of Pthl

This study

B. methylotrophicum/pXY1-LysA

B. methylotrophicum overexpressing the gene of LysA under the control of Pthl

This study

B. methylotrophicum/pXY1-DapAB

B. methylotrophicum overexpressing the gene of DapA and DapB under the control of Pthl

This study

Plasmids

pMCljS

pACYC184, methyltransferase gene of C. ljungdahlii (CLJU c03310), SpeR

[16]

pXY1

pCB102 ORI, ColE1 origin; AmpR, EmR

[16]

pXY1-Pthl-NikABCDE

Derived from pXY1, with the operon of nikA, nikB, nikC, nikD and nikE overexpression

This study

pXY1-Pthl-FhuBCD

Derived from pXY1, with the operon of FhuB, FhuC and FhuD overexpression

This study

pXY1-LysA

Derived from pXY1, with the gene of LysA overexpression

This study

pXY1-DapAB

Derived from pXY1, with the gene of DapA and DapB overexpression

This study

  1. SpeR, spectinomycin resistance; AmpR, ampicillin resistance; EmR, erythromycin resistance; pCB102 ORI, Gram-positive origin of replication from Clostridium butyricum