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Fig. 6 | Biotechnology for Biofuels and Bioproducts

Fig. 6

From: A novel sucrose-inducible expression system and its application for production of biomass-degrading enzymes in Aspergillus niger

Fig. 6

PfopA-mediated the β-glucosidase (BGL) gene from A. niger C112 (bgla) expression in A. niger. A Detection of BGL activities of A. niger strains on the CMC-esculin plate. FBL9 and FBL20 represented recombinant strains expressing bgla. 20611 represented the parental strain A. niger ATCC 20611. B Southern blot assay for confirmation of the bgla gene insertion in the genome of A. niger. C RT-qPCR analysis of the expression levels of bgla. Actin was used as a reference gene and the 2−ΔΔCt method was used for calculating relative expression levels. D SDS-PAGE analysis of the extracellular protein of FBL9, FBL20, and the parental strain ATCC 20611 (20611) on sucrose. E BGL activity of FBL9, FBL20, and the parental strain ATCC 20611 cultivated in the sucrose-containing medium after 3 d fermentation. BGL activity was detected using p-Nitrophenyl-β-d-glucopyranoside (pNPG) as the substrate. F Quantification of extracellular protein concentrations of FBL9, FBL20, and the parental strain ATCC 20611 (20611) by the Bradford method. Values and standard deviation (SD) of triplicates are presented in the figure. Asterisks indicate statistically significant differences (**p < 0.01) as assessed by Student’s t test. n.d., not detected

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