Fig. 3

Production of p-coumaric acid by Crabtree positive and negative S. cerevisiae. a Schematic representation of p-coumaric acid biosynthetic pathways in S. cerevisiae. Yeast native genes, including pentafunctional arom protein (ARO1), bifunctional chorismate synthase/riboflavin reductase (ARO2), 3-deoxy-7-phosphoheptulonate synthase (ARO3, ARO4), chorismate mutase (ARO7), aromatic aminotransferase I (ARO8), aromatic aminotransferase II (ARO9), and prephenate dehydrogenase (TYR1) are shown in orange. Tyrosine ammonia lyase (FjTAL) from Flavobacterium johnsoniae is shown in red. b Titer of p-coumaric acid produced by Crabtree positive 11C-PCA and Crabtree negative 28-PCA; c specific titer of p-coumaric acid in Crabtree positive 11C-PCA and Crabtree negative 28-PCA. *p value < 0.05