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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: A highly efficient transcriptome-based biosynthesis of non-ethanol chemicals in Crabtree negative Saccharomyces cerevisiae

Fig. 5

Production of 3-HP and free fatty acids by Crabtree positive and negative S. cerevisiae. a Schematic representation of 3-HP and free fatty acids biosynthetic pathway in S. cerevisiae. Yeast native genes, including acetyl-CoA carboxylase (ACC1), beta subunit of fatty acid synthetase (FAS1), alpha subunit of fatty acid synthetase (FAS2), peroxisomal acyl-CoA thioesterase (TES1), long chain fatty acyl-CoA synthetase (FAA1, FAA4), and fatty-acyl coenzyme A oxidase (POX1) are shown in orange. Malonyl-CoA reductase (CaMCR) from Chloroflexus aurantiacus is shown in red. b Titer of 3-HP produced by Crabtree positive 11C-HP and negative 28-HP; c Specific titer of 3-HP in Crabtree positive 11C-HP and negative 28-HP; c titer of FFA produced by Crabtree positive 11C-FFA, negative 28-FFA and 28-FFA-E; d specific titer of FFA in Crabtree positive 11C-FFA, negative 28-FFA and 28-FFA-E. ***p value < 0.001; **p value < 0.005; *p value < 0.05

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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