Fig. 6From: A highly efficient transcriptome-based biosynthesis of non-ethanol chemicals in Crabtree negative Saccharomyces cerevisiaeFed-batch fermentation of FFA in 28-FFA-E. Cultivation was carried out in minimal salt medium with pH 5.0 adjusted by KOH. The dissolved oxygen concentration was maintained above 20% saturation by automatic adjustment of the stirring rate. Glucose feeding was initiated at 40Â h. a FFA production, OD and glucose consumption by Crabtree negative 28-FFA-E. FFA titer is the sum of all detected FFA, including C8, C10, C12, C14, C16:1, C16, C18, C18:1; b production of pyruvate and citrate during fed-batch fermentation of FFA in Crabtree negative 28-FFA-EBack to article page