Table 1 Strains list in this study
Strains | Genotype | Â |
|---|---|---|
CEN.PK113-11C | MATa MAL2-8c SUC2 ura3-52 his3Δ1 | Kötter, University of Frankfurt, Germany |
CEN.PK YMZ-E1 | MATa ura3-52 his3-∆1 pdc1∆ pdc5∆ pdc6∆ | [77] |
sZJD-28 | CEN.PKYMZ-E1 acs2Δ::TEF1p-POav-ADH1t acs1Δ::TEF1p-PTAse-CYC1t gpp1Δ gpp2Δ X-2::KanMX-TEF1p-Cas9-CYC1t MED1*432Y GPD1W71* | [12] |
11C-BD | CEN.PK113-11C plasmid-pSP-GM2 | This study |
28-BD | sZJD-28 plasmid-pSP-GM2 | This study |
11C-BD-E | CEN.PK113-11C plasmid-2, 3-BD GM2 | This study |
28-BD-E | sZJD-28 plasmid-2, 3-BD GM2 | This study |
11C-LA | CEN.PK113-11C plasmid-LDH GM2 | This study |
28-LA | sZJD-28 plasmid -LDH GM2 | This study |
11C-PCA | CEN.PK113-11C XII-2::TEF1p-TAL-CYC1t | This study |
28-PCA | sZJD-28 XII-2::TEF1p-TAL-CYC1t | This study |
11C-Lyc | CEN.PK113-11C XII-2::CYC1t-CrtE-pCDC19-pCCW12-CrtB-ADH1t-TDH2t-CrtI-pTDH3 | This study |
28-Lyc | sZJD-28 XII-2::CYC1t-CrtE-pCDC19-pCCW12-CrtB-ADH1t-TDH2t-CrtI-pTDH3 | This study |
11C-Far | CEN.PK113-11C XII-2::ADH1t-FS-pTEF1 | This study |
28-Far | sZJD-28 XII-2::ADH1t-FS-pTEF1 | This study |
11C-HP | CEN.PK113-11C XII-2::TEF1p-MCR-CYC1t | This study |
28-HP | sZJD-28 XII-2::TEF1p-MCR-CYC1t | This study |
11C-FFA | CEN.PK113-11C pox1Δ faa1Δ faa4Δ | This study |
28-FFA | sZJD-28 pox1Δ faa1Δ faa4Δ | This study |
28-FFA-E | sZJD-28 pox1Δ faa1Δ faa4Δpah1Δ | This study |