Skip to main content
Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid

Fig. 2

Enhancing the supply of precursor succinyl-CoA for 5-ALA production. A Schematic diagram of enhancing succinyl-CoA supply via overexpressing key genes (bold blue arrows). B Engineered strains for 5-ALA production. Genetic elements on the plasmids: Ptrc, IPTG-inducible trc promoter; Ptet, aTc-inducible tet promoter; PJ23100, J23100 constitutive promoter; Pppc, ppc promoter; lacO, lac operator; tetO, tet operator. C 5-ALA titer, D yield and E biomass of engineered strains. Calcium pantothenate (0.2 g/L) was supplemented. Additional 10 g/L glucose and 2 g/L glycine were supplemented after 12 h cultivation. Data are presented as mean values +/− SD (= 3 independent experiments). **P < 0.01, *P < 0.05, Student’s two-tailed t-test

Back to article page