Fig. 6

Effects of IPTG and CWP on ATX3-Q55 production and cell physiology. Growth curves of cells overexpressing ATX3-Q55 upon induction with IPTG (A) and CWP (B). The cell density (full dots, black line, OD₆₀₀), and the concentration of glycerol (blue line) and lactose (empty dots, dotted black line) are plotted in function of growth times. The vertical dotted line indicates the addition of the inducer, error bars indicate the standard deviation of three independent measurements. C The production of ATX3-Q55 was monitored by WB analysis on crude extracts from samples taken at the indicated times. One of three independent WB images was shown. The effects of inducers are shown as the ratio of the amount of ATX3-Q55 produced with CWP to that produced with IPTG. The amount of ATX3-Q55 was estimated by densitometric analysis and normalized using PNPase signals. E Amino acid consumption determined by HPLC analysis of the culture supernatant from samples taken at the indicated times. F Cell viability determined by flow cytometry analysis after labeling of the whole cells with propidium iodine. F SOD specific activity measured by McCord and Fridovich assays on the soluble protein fraction extracted from samples taken at the indicated times. Mean values of three (D–F) or four (G) independent measurements are represented with error bars indicating standard deviations. Statistical analyses were performed using unpaired two-tailed Student’s t-test, n.s.: not significant p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001