Table 1 Plasmids and strains used in the present study
Designation | Genotype or description | Source |
|---|---|---|
Plasmids | ||
 pET-28a ( +) | KanR, PT7 | Novagen |
 pUC19 | AmpR, PT7 | Novagen |
 pE01 | pET-28a ( +) origin, KanR, PT7, tdiA–tdiE | This study |
 pE02 | pET-28a ( +) origin, KanR, PT7, tdiA–tdiE and sfp | This study |
 pU03 | pUC19 origin, AmpR, PT7, ScCK and AtIPK | This study |
Strains | ||
 BL21-AI | F-ompT gal dcm lon hsdSB(rB− mB−) araB::T7RNAP-tetA | Novagen |
 BL-control | BL21-AI/pET-28a ( +) | This study |
 BL-1 | BL21-AI/pE01 | This study |
 BL-2 | BL21-AI/pE02 | This study |
 BL-3 | BL21-AI/pE02/pU03 | This study |